Y, plus the data had been analysed using the Student’s t test. Organ weights were expressed because the mean SD of five animalsMem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 111(3), Marchper group and also the information had been analysed applying ANOVA, followed by Tukey post-test. Variations have been regarded important at p 0.05 (represented by an asterisk).RESULTSFingerprint of SSPHE – HPLC-DAD-MS/MS evaluation of SSPHE showed the presence of two classes of compounds: biflavonoids and caffeoyl-hexoside derivatives of higher molecular weight (Fig. 1A). The primary biflavonoids, amentoflavone, and robustaflavone were identified within a earlier operate (Rizk et al. 2014); now two new biflavonoids are observed (Table I). The peak at 34.three min with an m/zof537.0821 [M-H]- is compatible using the formula C30H18O10 (537.0827, error 1.2 ppm). The fragmentation of m/z 537 generates the ions at m/z 284 (C15H8O6) and m/z269 (C15H9O5). This peak was putatively identified as hinokiflavone depending on their fragments and UV spectrum along with the peak at 35.9 min m/z 551.0977 (compatible together with the formula C31H20O10 – 551.0984, error 1.3 ppm) showed a related UV spectrum and fragments of 34.three min and was putatively identified as OMe-hinokiflavone. Polar compounds among 11-17 min had been also observed within the chromatogram. These compounds showed an UV spectrum characteristic on the caffeoyl/feruloyl group (Grassi-Zampieron et al. 2010) as well as a molecular weight range of 990-1638 Da (Table I). The fragmentation patterns of those peaks are equivalent towards the sequential losses of caffeoyl acids and hexose moieties (Fig. 1B, C). The compounds had been putatively determined as di, tri, or tetracaffeoyl acids with tetra, penta, or hexahexosides,Fig. 1A: high-performance liquid chromatography-mass spectrometry chromatographic profile and ultraviolet absorption spectra with the polar hydroethanolic extract from Selaginella sellowii; B: fragmentation of your peak 12.Formula of 5-Chloro-1H-pyrazolo[4,3-d]pyrimidine eight min m/z 989.BuySucrose monolaurate 2788 with an power collision of 64.PMID:23551549 8 eV; C: fragmentation of the peak 13.3 min m/z 818.2241 with an power collision of 46.4 eV. 1: dicaffeoyl-O-tetra-hexoside; three: tetra-caffeoyl-O-hexa-hexoside; 5: tetra-caffeoylO-hexa-hexoside; six: tetra-caffeoyl-O-penta-hexoside; 7: amentoflavone; eight: robustaflavone; 9: hinokiflavone; ten: OMe-hinokiflavone.In vivo activity of S. sellowii Dayane Priscilla de Souza Queiroz et al.Retention occasions (Rt), maximal absorption wavelength (UV/VIS), formula, and molecular weight (m/z) of compounds from the polar hydroethanolic extract from Selaginella sellowii (SSPHE)827 (C33H47O24), 665 (C23H41O21), 503 (C18H31O16), 341 (C12H21O11), 161 (C9H5O3) putative dicaffeoyl-O-tetra-hexoside 827 (C33H47O24), 665 (C23H41O21), 503 (C18H31O16), 341 (C12H21O11), 161 (C9H5O3) putative dicaffeoyl-O-tetra-hexoside 1151 (C51H59O30 ), 989 (C42H53O27), 827 (C33H47O24), 161 (C9H5O3) putative tetra-caffeoyl-O-hexa-hexoside 989 (C42H53O27), 827 (C33H47O24), 665 (C23H41O21), 161 (C9H5O3) putative tri-caffeoyl-O-penta-hexoside 1151 (C51H59O30 ), 989 (C42H53O27), 827 (C33H47O24), 161 (C9H5O3) putative tetra-caffeoyl-O-hexa-hexoside 1151 (C51H59O30 ), 989 (C42H53O27), 827 (C33H47O24), 665 (C23H41O21), 161 (C9H5O3) putative tetra-caffeoyl-O-penta-hexoside 375 (C21H11O7), 331 (C20H11O5) amentoflavone 331 (C20H11O5), 309 (C17H9O6) robustaflavone 284 (C15H8O6), 269 (C15H9O5) putative hinokiflavone 283 (C15H7O6), 255 (C14H7O5) putative OMe-hinokiflavonebased around the molecular formula and fragmentation; on the other hand, the groups’ position couldn’t be determined. Mol.